Hydroxyurea is highly reactive compound. In my opinion it either reacts
with NAD or even with LDH itself. The mechanism of reaction probably
relies upon some kind of redox process. Comparison of standard redox
potential of hydroxyurea and standard redox potential of NAD can provide
some answers in relation to that. Hydroxyurea can also make covalent bonds
and that should be also kept in mind as well as reactivity of its
metabolic products. However, I am not absolutely sure that the compound
(or its metabolites) within therapeutic concentration range can make such
interference. Hope this helps.
Zeljko Debeljak, PhD
CROATIA
> Dear colleagues,
>
> Can I ask if anyone has experienced interference with their LDH assay (we
> use the Beckman Coulter DX) from anti-cancer drugs?
> We had a recent experience of a patient with an artefactually low LDH who
> had recently started treatment with hydroxyurea.
>
> Many thanks
>
> Dr. Sean Maguire,
> Biochemistry Laboratory,
> Mater Hospital,
> Dublin.
>
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