Sanjiv Kumar wrote:
> I have not tried purifying this protein either with detergent or with
> /Guanidine Hydrochloride. I can try with both /Guanidine HCl and
> detergent. Since I got good purification with the 8M urea, I was
> thinking if this protein could be refolded and used further. Isn't
> removal of detergent more difficult then Urea?
> Sanjiv Kumar
> Lab. No. 411,
> Functional Genomics Unit,
> Institute of Genomics and Integrative Biology,
> New Delhi-110007
> India
Is it a transmembrane protein or not? or just a protein associated to
the membrane (with a GPI anchor for example)?
If it is a transmembrane protein, you should try to use detergents for
extraction and during the purification.
David
--
David Cobessi
Institut de Biologie Structurale
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