Sorry Charlie for the late reply.
I'm using the CFX96 5 channel with Sypro and other dyes. I tested the
machine against the Eppendorf product nd decided to go for the Biorad
version as running identical samples gave better results on the
Biorad. This could be a machine specific problem but on a 96 well
plate the detection level was ~1.5x better, you can use the HEX
channel for detection.
Jürgen
On 29 Apr 2009, at 03:06, Charlie Bond wrote:
> Hi Folks,
>
> Has anyone here had any success running a thermofluor (differential
> scanning fluorimetry) experiment on a BioRad CFX96 with SYPRO orange
> as
> the fluorophore?
>
> Our local 'technical expert' tells us it should work and gave us some
> settings to test, but was very sketchy about any detail, and in a test
> we ran we got some strange results (every well that contains SYPRO
> orange - even those with no protein - have a peak in fluorescence at
> 58oC).
>
> Before we tinker, or go and persuade a friendly molbiol lab to let us
> play with the filters in their iq5, I thought I'd see if anyone here
> had
> any advice.
>
> I'll summarise any responses I get.
>
> Cheers,
> Charlie
>
> --
> Charlie Bond
> Professorial Fellow
> University of Western Australia
> School of Biomedical, Biomolecular and Chemical Sciences
> M310
> 35 Stirling Highway
> Crawley WA 6009
> Australia
> [log in to unmask]
> +61 8 6488 4406
-
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Biochemistry and Molecular Biology, W8708
615 North Wolfe Street
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-3655
http://web.me.com/bosch_lab/
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