Dear Remy,
Iodoacetamide might be a good candidate as it alkylates thiol groups and
is also used as cysteine protease inhibitor.
good luck,
hannes
-----
Hannes Uchtenhagen, Ph.D student
Karolinska Institutet
Center for Infectious Medicine (CIM)
Karolinska University Hospital Huddinge, F59
SE-141 86 Stockholm, Sweden
CIM: +46-(0)8-585 896 88
MTC: +46-(0)8-524 86216
Mobile: +46-(0)7-36901461
----- Original Message -----
From: Remy Loris <[log in to unmask]>
Date: Wednesday, January 28, 2009 2:47 pm
Subject: [ccp4bb] Off-topic: chemical modification on thiol groups
To: [log in to unmask]
> I am looking for a reagent (and vendor) that will irreversible put
> a
> raher bulky substituent on a free SH group and that does not react
> with
> free amines (or other potential reactive groups present on a
> protein
> surface). The connection with crystallography is that it is
> required for
> an experiment asked by a referee necessary to confirms or reject a
> hypothesis that results from a crystal structure. For this
> experiment it
> is essential that the reaction is not reversible (so no S-S bond
> formation).
>
> Remy Loris
> Vrije Universiteit Brussel
>
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