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Dear Remy, Iodoacetamide might be a good candidate as it alkylates thiol groups and is also used as cysteine protease inhibitor. good luck, hannes ----- Hannes Uchtenhagen, Ph.D student Karolinska Institutet Center for Infectious Medicine (CIM) Karolinska University Hospital Huddinge, F59 SE-141 86 Stockholm, Sweden CIM: +46-(0)8-585 896 88 MTC: +46-(0)8-524 86216 Mobile: +46-(0)7-36901461 ----- Original Message ----- From: Remy Loris <[log in to unmask]> Date: Wednesday, January 28, 2009 2:47 pm Subject: [ccp4bb] Off-topic: chemical modification on thiol groups To: [log in to unmask] > I am looking for a reagent (and vendor) that will irreversible put > a > raher bulky substituent on a free SH group and that does not react > with > free amines (or other potential reactive groups present on a > protein > surface). The connection with crystallography is that it is > required for > an experiment asked by a referee necessary to confirms or reject a > hypothesis that results from a crystal structure. For this > experiment it > is essential that the reaction is not reversible (so no S-S bond > formation). > > Remy Loris > Vrije Universiteit Brussel >