Transferrin saturation can be calculated directly from Iron and
Transferrin saturation can be calculated directly from Iron and transferrin (TRN) concentrations. 1 molecule of transferrin will bind two molecules of iron.
TRF % saturation = Fe / (2 x TRF) X 100
Fe is measured in µmol/l and TRF in g/l. The molecular mass of TRF is 79570daltons. To convert TRF to µmol/l divide by 0.07957.
The equation becomes:
TRF % saturation = Fe / (2 x TRF/ 0.07957) x 100
= Fe (µmol / l) / TRF (g / l) x 3.9785
The molecular mass source is :
CLIN.CHEM.38/10, 2078-2081 (1992)
2078 CLINICAL CHEMISTRY,Vol. 38, No. 10, 1992
Transferrin Index: An Alternative Method for Calculating the Iron Saturation of
Transferrin
John Beilby et al.
There is a further ref. for molecular mass in the above paper.
Mike Collins
BMS3
Biochemistry Automation
Norfolk & Norwich University Hospital
England
http://www.nnuh.nhs.uk/
-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Clare Jeffray
Sent: 19 November 2008 11:18
To: [log in to unmask]
Subject: Transferrin saturation
I am interested to hear from anyone providing a calculation of transferrin
saturation, from measurement of iron and transferrin, as to what molecular
weight they are using for transferrin in their calculation as references state
anywhere from 75 kDa to over 90kDa.
Many thanks
Clare
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