Dear Kristof,
I recently had lots of trouble with molrep in space group
F432. Both Phaser and Molrep failed, only Amore produced
a solution.
Hope that helps.
Manfred.
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On Tue, 13 Nov 2007, Kristof Van Hecke wrote:
> Dear all,
>
> When running Pointless 1.2.0 (see summary below), I get the cubic
> space group P 4 3 2
> (mosflm gave P 2 3 or P 4 3 2)
>
> <!--SUMMARY_BEGIN-->
>
> Best Solution space group P 4 3 2
> Reindex operator: [h,k,l]
> Laue group probability: 1.000
> Systematic absence probability: 0.975
> Total probability: 0.975
> Space group confidence: 0.964
> Laue group confidence 1.000
>
> <!--SUMMARY_END-->
>
> However, although I have multiple models (ensemble) with
> approximately 30% sequence identity (44% homology), I'm not able to
> get any reasonable solution with Phaser.
> As we know the protein can form dimers and even trimers, is it
> possible that Poinless is giving a higher space group because of
> pseudo-symmetry in this case..?
>
> Does anyone has got experience with Pointless and this kind of space
> group please..?
>
> Many thanks
>
> Kristof
>
>
> --------------------------------------
> Kristof Van Hecke, PhD
> Biomoleculaire Architectuur
> Celestijnenlaan 200 F
> B-3001 Heverlee (Leuven)
> Tel: +32(0)16327477
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