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Dear Kristof, I recently had lots of trouble with molrep in space group F432. Both Phaser and Molrep failed, only Amore produced a solution. Hope that helps. Manfred. ******************************************************************** * * * Dr. Manfred S. Weiss * * * * Team Leader * * * * EMBL Hamburg Outstation Fon: +49-40-89902-170 * * c/o DESY, Notkestr. 85 Fax: +49-40-89902-149 * * D-22603 Hamburg Email: [log in to unmask] * * GERMANY Web: www.embl-hamburg.de/~msweiss/ * * * ******************************************************************** On Tue, 13 Nov 2007, Kristof Van Hecke wrote: > Dear all, > > When running Pointless 1.2.0 (see summary below), I get the cubic > space group P 4 3 2 > (mosflm gave P 2 3 or P 4 3 2) > > <!--SUMMARY_BEGIN--> > > Best Solution space group P 4 3 2 > Reindex operator: [h,k,l] > Laue group probability: 1.000 > Systematic absence probability: 0.975 > Total probability: 0.975 > Space group confidence: 0.964 > Laue group confidence 1.000 > > <!--SUMMARY_END--> > > However, although I have multiple models (ensemble) with > approximately 30% sequence identity (44% homology), I'm not able to > get any reasonable solution with Phaser. > As we know the protein can form dimers and even trimers, is it > possible that Poinless is giving a higher space group because of > pseudo-symmetry in this case..? > > Does anyone has got experience with Pointless and this kind of space > group please..? > > Many thanks > > Kristof > > > -------------------------------------- > Kristof Van Hecke, PhD > Biomoleculaire Architectuur > Celestijnenlaan 200 F > B-3001 Heverlee (Leuven) > Tel: +32(0)16327477 > -------------------------------------- > > > > > > Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm > >