Quoting Edward Berry <[log in to unmask]>:
> Savvas Savvides wrote:
> > Indeed, but wouldn't consideration of micelle size affect our
> > estimation of the number of molecules in the asu, in some cases
> > significantly?
> Good point- I think now that is taken into account by just saying
> "membrane proteins tend to have a high solvent content" and taking
> that into consideration when you guess the number of molecules.
> But it would be nice to account for the detergent explicitly.
> Say by analyzing detergent content of the crystals, or in some
> ideal cases neutron diffraction with perdeuterated detergent.
>
with regard to this has anyone actually checked how the micelle properties
with or without protein "embedded" might differ?? are we assuming empty
micelle and the protein-added micelle are the same size/Mw? is this really
so?
--- of course this may further vary depending on the oligomeric state of the
protein --suppose some neutron scattering studies on model systems might
give the answer --havent looked. just wondering..
-tommi
--
Tommi Kajander, Ph.D.
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
PO box 65 (Street address: Viikinkaari 1, 4th floor)
University of Helsinki
FIN-00014 Helsinki, Finland
Tel. +358-9-191 58903
Fax +358-9-191 59940
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