Das, Debanu wrote:
> Hi,
> There are at least 4 methods to try to estimate amount of detergent in a membrane protein crystal
.............
>
> In summary, someone wanting to estimate amount of detergent in their
> crystals and have sufficiently large and numerous crystals, could try out
> any of the above methods. The above techniques are quite well documented
> in literature. The first 3 can be done in-house and so can FTIR.
I happen to have the reference for Ron Kaplan's TLC method at my fingertips:
Eriks, L. R., Mayor, J. A. & Kaplan, R. S. (2003). A strategy for
identification and quantification of detergents frequently used
in the purification of membrane proteins. Anal Biochem 323, 234-41.
Sensitivity is limited by the amount of aqueous solution you can spot on a TLC plate.
Probably could improve sensitivity by speed-vac-ing an aliquot to near dryness
and redissolving in 50% methanol or something.
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