a couple of points from long BC about approx. 0 C cooling...for what its worth!
i) don't forget that high concentrations of salts can have a significant effect on the freezing point ( remember freezing point depression?). We used to collect data at -15C from crystals grown in 2.4M Ammonium Sulphate
ii) cool slowly and gently over a couple of hours
iii) put the mother liquor at only the colder end of the capillary to prevent condensation
iv) we found a transition at about -5 for above crystals, so be careful.
Peter Moody
Henry Wellcome Laboratories of Structural Biology
Department of Biochemistry
Henry Wellcome Building
University of Leicester
LE1 9HN
0116 229 7097
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From: CCP4 bulletin board on behalf of mesters
Sent: Wed 11/07/2007 08:46
To: [log in to unmask]
Subject: Re: [ccp4bb] Help with reducing crystal mosaicity
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