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a couple of points from long BC about approx. 0 C cooling...for what its worth! i) don't forget that high concentrations of salts can have a significant effect on the freezing point ( remember freezing point depression?). We used to collect data at -15C from crystals grown in 2.4M Ammonium Sulphate ii) cool slowly and gently over a couple of hours iii) put the mother liquor at only the colder end of the capillary to prevent condensation iv) we found a transition at about -5 for above crystals, so be careful. Peter Moody Henry Wellcome Laboratories of Structural Biology Department of Biochemistry Henry Wellcome Building University of Leicester LE1 9HN 0116 229 7097 ________________________________ From: CCP4 bulletin board on behalf of mesters Sent: Wed 11/07/2007 08:46 To: [log in to unmask] Subject: Re: [ccp4bb] Help with reducing crystal mosaicity snipped