Dear Mailbase users
I am hoping someone can help with a problem we are having regarding serum Albumin measurement.
Currently we run Bromocresol Purple on our Beckman DxC800 'Urgent' lab analyser and Bromocresol Green on our Bayer Advia 2400 main analysers. Paediatrics are analysed using BCP on the Beckman and everyone else BCG.
This week two of our paediatric patients were analysed using BCG instead of BCP. In one patient instead of the value of 11g/L (this patient has nephrotic syndrome) that we had been getting with the BCP method, we obtained a value of 25 using BCG. In the second patient (12 year old road traffic accident) the albumin had been running at 26 with BCP and we got 34 with BCG. These differences were reproducible on checking previous samples.
We are aware of approximately a 4g/L difference in these methods and have double checked all QC (across the range), instrumentation, etc. We also found some information regarding heparin affecting dye-binding methods but this did not yield any answers. We have since analysed a number of patients on both methods and have not found the differences seen in these patients. This leads us to think that there may be a drug interference. However, the only common drug is paracetamol.
Has anyone else had experience of drug-related interferences seen in one method over the other? Does anyone else have any answers to why in these patients we are getting totally different results.
Thank you for your time
Hazel
Hazel Hutton
Clinical Biochemist
University Hospital of North Staffordshire
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