I have a paper that addresses some of the issues on the effect of delayed
centrifugation. A scanner copy in pdf form was sent to the mailbase on
Wednesday. It may not have been reached all members on the list as it is too
large a size for normal e-trafficking.
The article ref is: Delay in centrifugation and measurement of serum
constituents in normal subjects.
M. S. Devgun, in Clinical Physiology and Biochemistry, 1989; 7: 189-197
A copy of the pdf file is available on the jismail archive that can accessed
via the ACB web site. Alternatively, please contact me directly for a copy.
Manjit
As part of a transport review I am auditing the time taken from phlebotomy
to separation of serum from cells by centrifugation.
There is local discussion about which standard should be used for the audit.
Folklore would suggest that unseparated samples start to become unsuitable
at 4 to 6 hours (ignoring temperature and glucose for the moment) and the
laboratory makes a "judgement call" on samples received later than this
time. However Tietz states that "Plasma or serum should be separated from
cells as soon as possible, and certainly within 2 h", referenced to Laessig
et al, Am J Clin Pathol, 1976:66;598-604.
I would be grateful if anyone had references or evidence about which
standard we should be working to.
Regards
Martin Myers
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