I agree one cannot correlate lipaemic index which is based on turbidity with lipaemia. However, in relation to interference with most assays my understanding is that it is the turbidity that is the problem, not the concentration of lipid. Therefore the so-called lipaemic index (maybe calling it turbidity would be more appropriate) is more relevant than the level of lipaemia in determining which specimens are likely to give inaccurate results,
Damian
Dr Damian Griffin
Consultant Chemical Pathologist
Department of Clinical Biochemistry
University College Hospital,
Newcastle Road,
Galway,
Ireland
Tel: +353 (0)91 544825
Email: [log in to unmask]
-----Original Message-----
From: EDWARD KEARNEY [mailto:[log in to unmask]]
Sent: 22 December 2006 14:33
To: [log in to unmask]; Griffin, Damian, UCHG
Subject: Re: Total Bilirubin and lipaemia interference in Neonatal
samples
Can you correlate Lipaemic index which is based on turbidity with
Lipaemia.
Edward
Mr Edward M Kearney, MSc, MCB, FRCPath,
Consultant Clinical Scientist,
East Kent Hospitals NHS Trust,
Queen Elizabeth the Queen Mother Hospital,
St Peters Road,
Margate,
Kent,
CT9 4AN
>>> "Griffin, Damian, UCHG" <[log in to unmask]> 21/12/2006
12:44 >>>
There are two total bilirubin methods available on the Roche modular.
The standard method is interfered with at a lipaemic index of 75, but
the Bilirubin Total liquid method isn't interfered with until the
lipaemic index is 1000. The only snag with the liquid method to my
knowledge is that the it can't be used on lithium heparin samples,
Regards,
Damian Griffin
Dr Damian Griffin
Consultant Chemical Pathologist
Department of Clinical Biochemistry
University College Hospital,
Newcastle Road,
Galway,
Ireland
Tel: +353 (0)91 544825
Email: [log in to unmask]
-----Original Message-----
From: Clinical biochemistry discussion list
[mailto:[log in to unmask]]On Behalf Of Lang, Tim
Sent: 21 December 2006 10:01
To: [log in to unmask]
Subject: Total Bilirubin and lipaemia interference in Neonatal samples
Dear List,
I would like to know how other laboratories deal with lipaemic samples
with respect to total bilirubin assay. I am particularly interested
in
those labs who are supporting a SCBU or Neonatal ICU. In Belfast on
the
Roche, the lipaemic index is set at 75. As a lot of neonates are on
continuous TPN ( with intraalipid infusion) we are finding that a
proportion of sample results are being held back due to this level. A
lot of these babies are also on phototherapy treatment so the total
bilirubin result is essential.
I would be grateful to the views of the mailbase.
Thanks in advance.
Tim
Dr Tim Lang
Principal Clinical Scientist
Clinical Biochemistry Department
Kelvin Building
Royal Victoria Hospital
Belfast
BT12 6BA
02890 633064
02890 234029 (Fax)
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