Dear Marcel,
You can consider to mill the lyophilized specimen before extraction.
For DNA extraction, you had better dissect the fresh animals to have
clean tissues,
especially for colonial species.
Many organisms (e.g., copepods, amphipods, protozoans, etc.) are
associated with ascidians.
Ciao,
--
Prof. Euichi (Luigi) Hirose
Dpt. Chem., Biol. & Mar. Sci.,
Fac. Sci., Univ. Ryukyus.
Okinawa 903-0213, JAPAN
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