Hello Christopher
We use the same reagents (Sigma/ImmunoDiagnostics 'A' & 'B') as you,
but we do clean-up by precipitating calcium oxalate from ethanolic solution
after adjusting pH to 5.0-5.1, followed by dissolving in edta.
We get results for the Sigma controls which are quite low (75-80%) compared
to their quoted values.
If you want to check your method, you could try running it against this
method.
We are not a member of a QA program for urinary oxalate at the moment; could
you give me the contact details of your program?
Regards
Richard Scarff
Biochemistry Dept
Royal Perth Hospital
Western Australia.
-----Original Message-----
From: Christopher Wood [mailto:[log in to unmask]]
Sent: Wednesday, 31 December 2003 21:17
To: [log in to unmask]
Subject: Urine Oxalate
I am currently having problems with this assay using oxalate oxidase.
Reagents QC & prep tubes originally sourced via Sigma Diagnostics (R.I.P.)
Now obtained from another company that has taken over this business.
Internal QC is fine but last two external QA samples returned at approx *3
method mean. Only change has been activated charcoal tubes now provided by
new supplier. Rest of reagents & QC old Sigma stock sold on by new supplier.
Anyone else having similar problems?
Happy New Year
Chris Wood
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