Dear Joy,
I heard cocain is one of the best anesthetics for ascidians, but I have
never tried.
I usually use both menthol and MgCl2 for anesthesia.
I prepare MgCl2 solution that is roughly isosmotic to seawater, and mix with
seawater as 1:3 to 1:1. Then, add a few drops of menthol dissolved in
ethanol.
For anesthesia and dissection, you can consult with "Reef and shore fauna
of Hawaii, Section 6B: Ascidians (Urochordata)" by Abbot DP, Newberry AT,
Morris KM (Bishop Museum Press).
Ciao,
Luigi
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