Joy
Try the following: place the bowl with the relaxed animal in a freezer.
Once a thick crust of ice is formed, take it out, add the fixative and let
it de-frost in fixative.
It works with solitary and colonial ascidians. You only need to have them
relaxed in the first place.
Best wishes
Xavier
At 19:03 26/01/2004 +0000, you wrote:
>Hi there,
>
>Do any of you have some advice on dissecting solitary ascidians with a
>tough tunic such as Halocynthia pyriformis? Have any of you use
>narcotizing agents? Any adive would be appreciated.
>
>Joy Stacey
Xavier Turon
Dept. of Animal Biology (Invertebrates)
Fac. of Biology
Univ. of Barcelona
645, Diagonal Ave
08028 Barcelona
e-mail: [log in to unmask]
phone: 34-93-4021441
fax: 34-93-4035740
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