Greetings all,
re below, a couple of approaches I have used in the past for lowering amylase:
1. Ion exchange resin - Amylase requires Ca for activity (also renders the enzyme more susceptible to heat damage if you need an absolute kill...)
2. Insoluble cross linked starch - a rough affinity step - mix the (finely ground)insoluble starch into serum for 10-15 minutes, centrifuge and take off the serum supernatant - the bulk of the amylase goes down with the starch.
A disadvantage with this approach (depending on the exact nature of the starch) can be a significant elevation in serum glucose level.
Cheers,
Colin Horner
Senior Scientist
Biochemistry Dept.
St. Vincent's Hospital Melbourne
-----Original Message-----
From: dr. C.H.H. Schoenmakers [mailto:[log in to unmask]]
Sent: Friday, 9 May 2003 12:04 AM
To: [log in to unmask]
Subject: Amylase inhibitor
Dear colleague,
Does anybody know how to inhibit amylase completely in combination with keeping the
sample matrix intact?
In order to make a control material to check our erythrocyte sedimentation rate we
added eloHAES, an artificial starch-based colloid, to blood. This works fine for some
hours, after which amylase in the sample has degraded the eloHAES. Therefore I would
like to eliminate amylase activity.
Best regards,
Christian
dr. C.H.H. Schoenmakers, European Clinical Chemist
Department of Clinical Chemistry, Elkerliek Hospital
P.O. box 98, 5700 AB Helmond
The Netherlands
(+)31-492-595555 (tel), (+)31-492-595691 (fax)
[log in to unmask]
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