My colleague, Graham Icke, who is not on the list has asked me to broadcast this information about his direct thiamine assay.
-----Original Message-----
From: Icke, Graham
Sent: Monday, 17 November 2003 6:18
To: [log in to unmask]
Subject: Thiamine Assay
Hi Graham,
Your request for details of a direct thiamine assay has been forwarded to me as we have been using a direct assay for thiamine, that I set up around 25 years ago.
The assay is a microbiological one which uses a chloramphenicol strain of Lactobacillus fermenti as the test organism. Being chloramphenicol resistant means that we can add this antibiotic to the test medium to prevent contamination without resorting to aseptic techniques and can use automated equipment for setting up and reading (after 22 hours incubation).
The assay is suitable for either serum (reflects recent intake of the vitamin) or red cells (reflects body stores) and we would assay around 100-150 samples per week in these laboratories.
The assay was set up as part of my Masters degree (1980) and I eventually got round to having the method published in 1994: J Clin Path 47:639-641. Automated microbiological assay of thiamin in serum and red cells: G Icke & D Nicol.
Please feel free to contact me if you require any further info.
Have a great day.
Kind Regards
Graham
Graham ICKE
Principal Scientist
Division of Laboratory Medicine
Royal Perth Hospital
Wellington St
Perth
Western Australia 6000
Telephone: 61 08 9224 2214
Fax: 61 08 9224 2491
E-Mail: [log in to unmask]
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