I remember the immense amount of work that went into defining both the
National and IFCC Standardised assays, so that there could be repeatablity
of enzyme assays (potentially) globally. This is, of course, a subset of the
old argument as to whether you should measure the biological activity of a
protein (e.g a hormone) or its mass.
As has been pointed out, manufacturers assays don't strictly conform to the
IFCC standard method, but are "adjusted" to give comparable results. I have
an uncomfortable feeling that this is a move dictated by convenience and not
by scientific considerations.
Perhaps Olympus could make a comment.
> -----Original Message-----
> From: KATHARINE HAYDEN [SMTP:[log in to unmask]]
> Sent: 10 November 2003 11:49
> To: [log in to unmask]
> Subject: Olympus enzyme calibration
>
> As users of the AU2700/640 will be aware, Olympus are moving to
> calibration for all of their enzyme assays. After 30 plus years of
> measuring the activity of enzymes by methods based on a factor derived
> from the molar extinction coefficient and standardised assay conditions,
> it seems like a sea change to move to calibration of enzymes as if they
> were albumin or glucose.
>
> I wondered if anyone had comments on this proposed change as I haven't
> seen any scientific arguments for or against it.
>
> Kath Hayden
> Principal Biochemist
> University Hospital Aintree
> Liverpool
>
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