Dear Clara Henig,
We no longer have AAS for Zn, but we have fewer problems now with a
"5-Br-PAPS" reagent (from Audit / Biostat) for zinc in our Dimension
analysers, using only serum from Sarstedt plain tubes.
We used to buy a copper reagent from the same suppliers. However they
changed the constituents (something to do with gelling), since when neither
their nor any other reagents we have tried can give a valid colorimetric Cu
assay in our hands.
Our hepatologists & neurologists have reluctantly agreed to using serum
caeruloplasmin assay alone as the first screen for "?Wilson's", then going
on to urines (&/or liver) analyses for higher suspicions. They cannot find
any "evidenced-based" papers which say that serum copper is essential in
this context!
Best wishes,
Les Culank
> Reply-To: "Dr. Clara Henig" <[log in to unmask]>
> Date: Sun, 20 Apr 2003 08:45:37 +0100
> To: [log in to unmask]
> Subject: colorimetric assay for Cu and Zn
>
> Dear all,
>
> Does anyone use a colorimetric assay for measuring Cu or Zn?
> I will be happy to know how does this assay relate with the "gold
> standard" - atomic absorption?
>
> thank you in advance,
>
> Dr. Clara Henig
> Biochemistry departement
> Regional Laboratory of North Israel
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