Problem solved (mostly)
Dear All
Alas the excitement is no longer as intense.
Thanks for all those who responded.
Suspects offered were gelofusine and aminoglycoside antibiotics.
Patient had been on cefradine, but not aminoglycosides, and gelofusin was
mentioned but nobody could remember how much or when. Examination of notes
revealed 1 litre of gelofusin administered midway through the 24 hour
collection.
Summary of results :
"total protein"
pyrogallol red (Olympus AU640) 5184 mg/L
benzethonium chloride (Roche 917) 357 mg/L
pyrocatechol violet-molyb/ox (Vitros) 330 mg/l
turbidimetric albumin (Olympus AU640) 69.6 mg/l
urine dipstick (Bayer) trace
shake the bottle vigorously unstable foaming
urine electrophoresis see attached
I am not convinced that the other methods don't also have interference,
since the albumin and "protein" concentrations are so discrepant.
I have aliquots of the urine in the freezer if anyone would like to play.
with best wishes
Richard
Richard Mainwaring-Burton
Consultant Biochemist
Queen Mary's Hospital
Sidcup, Kent
DA14 6LT
020-8308-3084
-----Original Message-----
From: Mainwaring-Burton Richard (RGZ)
Sent: 29 September 2003 11:55
To: [log in to unmask]
Subject: pyrogallol red interference
Please can any member recall or inform of any known interference in
pyrogallol red method for urine/CSF protein ?
Over the weekend we had a urine from an antenatal (31 wks) ?Pre-eclampsia
lady which gave following investigations
pyrogallol red (Olympus AU640) 5 g/L
microalbumin (Olympus AU640) 70 mg/L
Bayer multistix trace +
vigorous shake unstable foam
Immediate ? relevant PMH = UTI treated with Cefradine
with best wishes
Richard
Richard Mainwaring-Burton
Consultant Biochemist
Queen Mary's Hospital
Sidcup, Kent
DA14 6LT
020-8308-3084
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