Leaving samples for SPE on the bench untill analysis will still cause
problems
1. with samples with cryoglobulins that precipitate rather rapidly. We have
been losing cryo's in samples that were cooled during clotting just a little
below 37 C (published in Clin Chem Lab Med 2003;41:85-89); such samples also
may cause problems like the one shown by Dave Ricketts.
2. complement will be degraded when samples will be left on the bench. Such
problems will be seen when the time between sampling and analysis increases
to longer then 24 hours. Such problems may be encountered when using
machines that can analyse large series of samples at the same time.
Andries
Dr. A. J. Bakker, Klinisch chemicus,
St. Klinisch Chemisch Laboratorium,
Postbus 850,
8901 BR LEEUWARDEN.
Tel.: 058-2888444
Fax: 058-2882227
E-mail: [log in to unmask]
> -----Oorspronkelijk bericht-----
> Van: Greg Watts [SMTP:[log in to unmask]]
> Verzonden: dinsdag 12 augustus 2003 1:22
> Aan: [log in to unmask]
> Onderwerp: Re: Cryoglobulins in Electrophoresis
>
> I was wondering why do we put EPG's into the fridge before analysis. I
> would
> have thought that it is better to leave them on the bench until analysis
> to
> avoid much of this problem with cryoglobulins.
>
> Greg
>
> Greg Watts
> Senior Biochemist
> Sydney Adventist Hospital
> (02) 9487-9518
>
>
> -----Original Message-----
> From: # David Ricketts [mailto:[log in to unmask]]
>
> Dear All
>
> Thanks for you input into our IgM issues. It was indeed a cryoglobulin.
> The
> patient was in clinic, clinically well and cryoglobulin was not suspected.
> We have amended our sops and will no longer run our Ep straight from the
> fridge.
>
> David Ricketts
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