Before I look at a gel, I always take a look at the albumin bands (including
all normal samples). Since we always measure albumin quantitatively before
electrophoresis, the albumin band should compare with other alb bands with
similar concentrations. If there is loading problem due to high viscosity,
you can pick it up this way. However, if you have cryoglobulin precipitated
before loading, you wouldn't pick it up. You'll have to depend on the tech
to inspect the serum tube before pipetting, another small but vital addition
to the SOP!
Hope my 2 cents helps,
PC
PC Chan, PhD, DABCC, FCACB
Clinical Biochemist,
Division of Clinical Biochemistry & Genetics,
Department of Clinical Pathology,
Sunnybrook & Women's College Health Sciences Center,
Rm CG68a, 2075 Bayview Avenue, Toronto M4N 3M5
Phone:416-4806100 ext 2688 fax: 416-4806120
Pager: 416-2359879 (internal 6805)
e-mail: [log in to unmask]
Lecturer, Department of Laboratory Medicine & Pathobiology, University of
Toronto
-----Original Message-----
From: # David Ricketts [mailto:[log in to unmask]]
Sent: Friday, August 08, 2003 9:18 AM
To: [log in to unmask]
Subject: IGM and scanning
Dear All
Advice please. We have recently had a patient with an IgM K paraprotein. We
ran the strip on Sebia HYDRASYS and then scanned the band and got twice
paraprotein levels of 5-6 g/l, with a globulin of 63. The next time the
patient presented, the sample was very viscous and would not apply. We then
treated the sample with a diluent, Fluidil, this then ran and the scanned
value was now 41 g/l. We have referred the sample to a protein reference
unit. We are obviously concerned that we have missed an opportunity to
intervene earlier.
Could we have any advice on protocols to avoid this happening again and /or
has anyone experienced anything like this before.
Many thanks
David Ricketts
Laboratory Manager North Middlesex University Hospital NHS Trust
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