Dear Michael,
I have myself tried to answer that question and we found, that fluoride has
no effect on the (in)stability of pyruvate in whole blood/plasma. Fluoride
inhibits certain steps in the glycolysis, but it will not inhibit LDH. Since
the equilibrium of this reaction is almost completely at the side of
lactate, all pyruvate will rapidly be converted into lactate. Our conclusion
was that only immediate deproteinisation with icecold perchloric acid wil
give satifying results.
With regards,
Bart Ballieux
Dr. B.E.P.B. Ballieux
Clinical Biochemist
Leiden University Medical Centre
P.O.box 9600
2300 RC Leiden
The Netherlands
Tel:+3171-5262165/2278
Fax: +3171-5266753
email: [log in to unmask]
-----Original Message-----
From: Dr. M. Steiner [mailto:[log in to unmask]]
Sent: dinsdag 3 juni 2003 12:28
To: [log in to unmask]
Subject: Blood collection and storage for pyruvate analysis
Dear colleagues,
We have got a request to run several hundreds of pyruvate analyses for
some "Biochemistry of exercise" project.
I fully realise that pyruvate is extremely unstable in blood. What I
wonder about is if we indeed will need to use some acid to immediately
precipitate proteins. This might be hard to accomplish for logistic
reasons. Would fluoride-containing blood tubes be an alternative with
the idea to stop lactate generation (and stabilize pyruvate levels,
too)? Still, I am not sure if pyruvate analysis will be reliable under
these conditions?
I am sure there are "old papers" on this which I have never seen nor
read ...
Thank you very much for your input.
Dr. med. Michael Steiner
University of Rostock
Institute of Clinical Chemistry & Pathobiochemistry
D-18057 Rostock
GERMANY
------ACB discussion List Information--------
This is an open discussion list for the academic and clinical
community working in clinical biochemistry.
Please note, archived messages are public and can be viewed
via the internet. Views expressed are those of the individual and
they are responsible for all message content.
ACB Web Site
http://www.acb.org.uk
List Archives
http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
List Instructions (How to leave etc.)
http://www.jiscmail.ac.uk/
------ACB discussion List Information--------
This is an open discussion list for the academic and clinical
community working in clinical biochemistry.
Please note, archived messages are public and can be viewed
via the internet. Views expressed are those of the individual and
they are responsible for all message content.
ACB Web Site
http://www.acb.org.uk
List Archives
http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
List Instructions (How to leave etc.)
http://www.jiscmail.ac.uk/
|