I have a similar concern about the Sebia system because a specimen
with cryoglobulin precipitated on the wick, and did not get applied to the
gel. The Sebia system provides very clean electrophoretic patterns because
it filters sera through a wick as the sample is applied to the gel. Based
on the note from David Ricketts and our experience, it seems that both
highly viscous samples and cryoglobulins may be trapped at the wick and
subsequently not applied to the gel, intermittantly.
regards, Andrew
Dr. Andrew W. Lyon, Clinical Biochemist / Home Office, Calgary, Canada.
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