Ian,
One year ago, we tried four different pyrogallol red urine protein methods
(Roche, Biomerieux, Olympus, Randox) before adopting the Randox reagent.
This choice was based on two antagonistic criteria
1) The reagent must correctly measure Ig free light chains
2) The interference of gelatin (plasma expanders) must be minimized : With
randox reagent, a standard treatment with plasmion may increase urine
proteins up to 0.5 g/l but this is not an unacceptable interference for a
patient in an intensive care unit.
The other reagents either underestimated light chains or had an increased
interference with gelatin. In fact, I think that the good choice depends on
the recruitement of patients.
Philippe Marquis
Centre hospitalier regional
Metz - France
www.multiqc.com
----- Original Message -----
From: "Ian Barlow" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Thursday, July 10, 2003 11:11 AM
Subject: Pyrogallol urine protein methods
> Dear colleagues,
> The manufacturer of our pyrogallol red urine protein method has recently
> changed the formulation of the reagent (regrettably) such that it is now
> susceptible to substantial positive interference from plasma expanders (in
> particular Gelofusine). Does anyone know of a pyrogallol urine protein
> method which is not affected by this?
> Many thanks.
>
> Ian Barlow
> Scunthorpe
> UK
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