I think it will be clinically apparent....
Assay doesn't look that difficult:
Determination of thiodiglycol in groundwater using solid-phase extraction
followed by gas chromatography with mass spectrometric detection in the
selected-ion mode. Tomkins, B. A.; Sega, G. A. Organic Chemistry and
Separations Section, Chemical and Analytical Sciences Division, Oak Ridge
National Laboratory, Oak Ridge, TN, USA. Journal of Chromatography, A
(2001), 911(1), 85-96. CODEN: JCRAEY ISSN: 0021-9673. Journal written
in English. CAN 134:167917 AN 2001:97935 CAPLUS
Abstract
A highly sensitive anal. procedure is described for detg. thiodiglycol in
groundwater. Samples are initially fortified with 3,3'-thiodipropanol
(surrogate), then both species are extd. using sequential solid-phase extn.
with both C18 and Ambersorb 572 columns. The C18 column, which removes
extraneous groundwater components, is discarded; the Ambersorb 572 column is
dried thoroughly before eluting polar components with a small vol. of
dichloromethane. The ext. is taken to dryness using dry flowing N2, and the
resulting residue is derivatized using
N-(tert.-butyldimethylsilyl)-N-methyltrifluoroacetamide and pyridine. The
derivatized products are dild. to a final vol. with toluene, chromatographed
using a fused-silica capillary column, and detected with a quadrupole mass
spectrometric detector in its selected-ion mode. Two independent,
statistically unbiased, procedures were used to evaluate the detection
limits for thiodiglycol; the values ranged between 4 and 16 mg L-1
groundwater.
Quantification of thiodiglycol in human urine after an accidental sulfur
mustard exposure. Jakubowski, E. M.; Sidell, F. R.; Evans, R. A.;
Carter, M. A.; Keeler, J. R.; McMonagle, J. D.; Swift, A.; Smith, J. R.;
Dolzine, T. W. EAI Corporation, Abingdon, MD, USA. Toxicology
Methods (2000), 10(2), 143-150. CODEN: TOMEEB ISSN: 1051-7235. Journal
written in English. CAN 133:130964 AN 2000:373187 CAPLUS
Abstract
A case of accidental human exposure to sulfur mustard, the chem. warfare
agent, was documented. The exposure produced skin damage in the form of
erythema and blisters comprising less than 5% of the body surface area.
Anal. methods verified and quant. analyzed the exposure by observing urinary
thiodiglycol levels using gas chromatog.-mass spectrometry. A max.
thiodiglycol urinary excretion rate of 20 mg / day was reached at day 3.
Concns. over 10 ng / mL were seen for as long as a week postexposure using
the std. assay. First-order anal. of the elimination data yielded a
thiodiglycol half-life of 1.2 d.
One of the earlier techniques involved converting TDG back to Mustard in a
head space vial...
Robert Forrest.
> -----Original Message-----
> From: Clinical biochemistry discussion list
> [mailto:[log in to unmask]]On Behalf Of Corns, Cathryn
> Sent: 07 February 2003 17:50
> To: [log in to unmask]
> Subject: Chemical weapons
>
>
> I've just been reading the DoH 'Guidelines for action in the
> event of a deliberate release' for various chemical agents. In
> the one on Mustard Gas the guidelines say that it is possible to
> confirm exposure to this agent by measuring the urinary
> metabolite, thiodiglycol. However, this isn't on Assay Finder -
> does anyone measure it? (Just in case of an unlikely event.....)
>
> If people aren't aware of this series of documents (which are
> quite useful, and include info relevant to laboratories) they can
> be found at: http://www.phls.org.uk/topics_az/deliberate_release/menu.htm
>
> Cathryn Corns
> Head of Biochemistry
> Southend Hospital
> 01702 435555 ext 4058
>
>
>
>
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