There is a paper from Clin Chem (1989;35/2:315-17) by Chan/Swaminathan/Cockram showing the effect of sodium fluoride as a preservative of glucose in whole blood and plasma. There is little difference within 60 minutes between fluoride and non-fluoride treated samples - glucose then appears to stabilise in plasma with fluoride added but there continues to be a gradual decline in whole blood treated with fluoride. There is a continual fall in both samples without fluoride.
Gareth Davies
Wrexham Maelor Hospital
N Wales
> -----Original Message-----
> From: Hogan, Tim [SMTP:[log in to unmask]]
> Sent: 05 September 2002 10:20
> To: [log in to unmask]
> Subject: Re: fluoride samples
>
> I have old data showing that delays of up to 7 hrs showed little clinical
> difference between fluoride and gel tubes, however this was from GP patients
> which all had normal white cell counts. In practice we only ask for fluoride
> samples on samples taken at GP surgeries and outlying hospitals, and for
> glucose tolerance tests, on the basis that the majority of samples taken
> within the hospital will have been centrifuged within 2hours of being taken.
> We also insist on fluoride samples from our neonatal unit as they seem to
> have a much higher rate of glucose utilisation.
>
>
> Tim
> Basildon
> [log in to unmask]
>
> -----Original Message-----
> From: p=NHS NATIONAL
> INT;a=NHS;c=GB;dda:RFC-822=ACB-CLIN-CHEM-GEN(a)JISCMAIL.AC.UK;
> Sent: 04 September 2002 16:58
> To: p=NHS NATIONAL
> INT;a=NHS;c=GB;dda:RFC-822=ACB-CLIN-CHEM-GEN(a)JISCMAIL.AC.UK;
> Subject:
>
>
> Glucose and fluoride oxalate - or not
> With the exception of blood for glucose tolerance tests I would be
> interested to know of any policies for plasma/serum glucose specimen
> collection. It is said - "Most labs, these days, use Gel separation
> tubes for Glucose and not fluoride oxalate" Is this true ?
> The main reason seems to be it's easier and cheaper to use a single tube for
> all chemistry tests. We have shown that glucose is stable for about 6 days
> at +4 after centrifuging but what about delay, temperature etc before
> spinning ? Should I insist upon fluoride oxalate ?
> I would like the views of a wider audience.
>
> Robin Barfoot - Clinical Scientist - Whipps Cross University Hospital,
> London. UK
>
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