Whole blood glucose declines by up to 0.5 mmol/L/h. Without
preservative it continues at the same rate to zero. In fluoride
oxalate tubes the decline stops after 1-2 hr and glucose is then
stable.
I agree with Mohamed that centrifugation within 1 hour of collection
makes serum/hep plasma acceptable, but for any longer times, fluoride
oxalate preservation is essential.
Bruce Muller
Charing Cross Hospital
London.
>>> "Barfoot Robin (RGC) Clinical Scientist - Patholgy"
<[log in to unmask]> 09/04/02 04:12pm >>>
Glucose and fluoride oxalate - or not
With the exception of blood for glucose tolerance tests I would be
interested to know of any policies for plasma/serum glucose specimen
collection. It is said - "Most labs, these days, use Gel
separation
tubes for Glucose and not fluoride oxalate" Is this true ?
The main reason seems to be it's easier and cheaper to use a single
tube for
all chemistry tests. We have shown that glucose is stable for about 6
days
at +4 after centrifuging but what about delay, temperature etc before
spinning ? Should I insist upon fluoride oxalate ?
I would like the views of a wider audience.
Robin Barfoot - Clinical Scientist - Whipps Cross University Hospital,
London. UK
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