Paul
Pardon my ignorance of the structure of E2 !!!!! - but you know what I mean.
Some assays detect only some of the oestrogen forms while others do not.
Ovarian cysts don't usually secrete significant levels of oestogen - and in
any case we have seen some women with 50 fold increases of E2 (not 3 fold).
Seems very puzzling - It's a number of years ago, but I am sure we assayed
one of these samples on Immulite and it came out normal.
regards
Bernie Croal
-----Original Message-----
From: Paul Masters [mailto:[log in to unmask]]
Sent: 12 July 2002 15:59
To: [log in to unmask]; Bernie Croal
Cc: Paul Masters
Subject: Re: TAMOXIFEN
On Thu, 11 Jul 2002 19:01:20 +0100, Bernie Croal
<[log in to unmask]> wrote:
>I suspect there may be an element of
>assay cross reactivity if the assay is direct to common amino acid
>sequences on the oestradiol and Tamoxifen molecules.
Amino acids? In a steroid molecule??!
The datasheet compendium for Nolvadex (tamoxifen) states, "..is a
non-steroidal, triphenylethelene-based drug which displays a complex
spectrum of oestrogen agonist and antagonist effects in different tissues."
Experiments to demonstate cross-reactivity are likely, therefore, to be
a waste of time and effort.
It is recognised to increase serum E2 levels in both pre- and
post-menopausal women, by about a factor of 3. Ovarian cysts have been
reported in
up to 80% of premenopausal women on treatmen (vs 8% of matched controls).
Paul Masters
Chesterfield
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