No, CK-BB or CK-MM are not macro-CKs. Macro-CKs are either CK associated
with immunoglobulines (macro-CK1), or mitochondrial/autoaggregated CK (macro
-CK2).
See other comment there: http://www.bmj.com/cgi/eletters/323/7315/705#EL3
>From: Bruce Campbell <[log in to unmask]>
>Reply-To: [log in to unmask]
>To: [log in to unmask]
>Subject: Re: Macro CK
>Date: Thu, 11 Oct 2001 09:50:02 +1000
>
>Since we lost access to CK electrophoresis we usually resolve this problem
>as follows. Do apparent CK-MB by immunoihibition. Since nearly all
>macroCKs are CK-BB this is usually elevated e.g. >25%. Second thing is to
>measure CK-MB by a mass assay. This should be normal. If it is a CK-MM
>macroenzyme which is rare, it becomes more difficult. If you can't access
>CK electrophoresis you can try PEG precipitation or gel filtration if you
>have the time and energy. Also consider polymyositis.
>
>Bruce Campbell
>
>****************************************
>Bruce Campbell FRCPA FAACB
>Sullivan Nicolaides Pathology
>Ph 61 (0)7 3377 8672
>Fax 61 (0)7 3870 5989
>Email [log in to unmask]
>****************************************
>
>
>
> Ian Godber
> <[log in to unmask]> To:
>[log in to unmask]
> Sent by: This list is an cc:
> open discussion list for Subject: Macro CK
> the academic community
> working in
> <ACB-CLIN-CHEM-GEN@JISCM
> AIL.AC.UK>
>
>
> 11/10/01 02:30
> Please respond to Ian
> Godber
>
>
>
>
>
>We've got a patient (45 year old female) with recurrent chest pain who has
>no ECG changes, negative Trop I and no other typical signs or symptoms of
>ischemic heart disease. She does however have a persistently raised CK
>(400-450 U/L). Does anyone offer a service for measuring macro CK and/or CK
>isozoenzyme electrophoresis to investigate her further?
>
>Ian
>
>Dr Ian Godber
>Clinical Chemistry
>Nottingham City Hospital
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