We use a working standard containing equal amounts of glucose, galactose and
fructose as the important hexoses, xylose as a representative pentose and
lactose as the most common disaccharide. We keep solutions of other sugars
available for co-chromatography to identify anything else. We also have a
photograph in the SOP of a plate prepared with all these sugars (in 4 lanes)
which shows the relative positions and colours. very useful when trying to
decide what to try for co-chrom.
Standards are made in 10% isopropanol and keep well at 4'C.
We use the same working standard with all sample types.
> Elizabeth Hall, Biochemist
> Department of Clinical Pathology,
> Royal Sussex County Hospital,
> Eastern Road,
> Brighton BN2 5BE
> T 01273 696955 x4148
> F 01273 664405
> E [log in to unmask]
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> ----------
> From: Jonathan
> Kay[SMTP:[log in to unmask]]
> Reply To: Jonathan Kay
> Sent: 23 May 2001 13:55
> To: [log in to unmask]
> Subject: Sugar chromatography; advice please
>
> What compounds should be in the standards for sugar chromatography of
> urine for clinical purposes.
>
> Ditto faeces?
>
> Dr Jonathan Kay
>
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