I would guess this observation reflects the size heterogeneity of
triglyceride-rich lipoproteins.
Seasonal greetings.
WM
At 08:01 20/12/2001 -0500, James Miller wrote:
>Graham Jones commented "...The correlation of the lipaemia index (on
>Hitachi) with amounts of triglycerides is poor, especially at relatively
>low concentrations (eg TG<10 mmol/L) and this is indicated by Roche in
>their literature. ..."
>
>This is true in my experience too. For a specific project in which
>lipemia and/or turbidity was an important issue, I recently set up a
>semiquantitative "turbidity index" by printing a series of numbers (0-9)
>with increasing pixel density and determining the smallest number I could
>make out through the serum/plasma in a standard tube size (the old read
>through estimate). Each sample was assayed for triglyceride and serum
>indices. Interestingly, the lipemia (L) index correlated fairly well with
>the "T-index," but correlated very poorly with triglyceride. In addition,
>the T-index (and L-index) were much better than triglyceride at predicting
>interference in the assay under study.
>
>I'm not sure how to explain this though. Are there other components of
>serum or plasma besides lipoproteins (thus triglycerides) that cause
>turbidity? Or is the variability of triglycerides in lipoproteins
>relative to their ability to scatter light so great that triglyceride is
>not a good measure of lipoprotein induced turbidity?
>
>-Jim
>
>James J Miller, Ph.D., DABCC, FACB
>Associate Professor
>Dept. of Pathology & Laboratory Medicine
>University of Louisville
>Louisville, KY 40292
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>
>502-852-1179
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