Hi Sonja,
and what about water followed by ethylacetate?
Best,
Bruno
-----Original Message-----
From: Collaborative Computational Project in Electron cryo-Microscopy <[log in to unmask]> On Behalf Of Dokland, Terje
Sent: lundi 22 avril 2024 20:19
To: [log in to unmask]
Subject: Re: [ccpem] washing Quantifoil grids with MetOH-stabilised chloroform?
Hi Sonja,
If I wash the grids at all, I usually use a wash with chloroform followed by a wash in ethanol, so I’m sure ethanol-stabilized chloroform should work just fine.
Terje
> On Apr 22, 2024, at 12:13 PM, Sonja Welsch <[log in to unmask]> wrote:
>
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> Dear Quantifoil-grid users,
>
> is anyone out there who can share their experience in washing QF grids
> (any type) for cryoEM SPA experiments with methanol- or
> ethanol-stabilised chloroform instead of "pure" chloroform?
>
> For some reason our lab chemical suppliers don't seem to be able to
> ship
> >99% chloroform without stabiliser anymore. I don't really think
> methanol or ethanol should be harmful to grids, but I'm no chemist and
> wonder if a stabiliser that potentially changes the polarity of a
> solvent might change grid "behaviour" in the cryoEM SPA sample prep
> process... Has anyone tested that and is willing to share their
> findings? (so our local users don't have to)
>
> Best regards,
>
> Sonja
>
>
> --
> Sonja Welsch
> Head of Central Electron Microscopy Facility Max Planck Institute of
> Biophysics Max-von-Laue-Strasse 3
> 60438 Frankfurt am Main, Germany
> Email: [log in to unmask]
> Mobile: +49 157 80625946
> Phone: + 49 69 6303 3015
>
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