Hi Daniel,
Yes, it would scale more or less with the fraction of white pixels in the
mask used for the focused classification.
HTH,
Sjors
> Hi all,
>
> In [Scheres et al. (2016) Methods in Enzymology. 579:125-157], it says
> that "one typically uses higher values of the regularization parameter T
> in masked classifications ... values of 10-40 have been used ...".
> Personally, I've seen improved results with values all the way up to
> several hundreds in focused, signal-subtracted 3D classification without
> alignment when the mask is very small relative to the entire box. Are
> there any general rule of thumb what values to try? E.g. in relationship
> to the fraction of the total box volume and/or total density included in
> the mask?
>
> Regards,
> Daniel
>
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--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
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