This is very protein-specific, for some proteins it is better to
co-crystallize with an inhibitor, then countersoak with a different
inhibitor, yet for others it is better to co-crystallize with an inhibitor
of interest directly. For all it's worth, I personally am a proponent of
the second approach, since soaking can and does generate scary artifacts.
Artem
> Hi, all,
> I met some crystal structures with disordered active sites. Soaking
> common ligands can not make it become ordered. I am wondering what
> people generally do in such situation.
>
> Thanks,
>
> Nian Huang
>
|