Are you using TLS?
If so I usually start with all Bs reset to the Wilson B ( see GUI) It
can still happen but not so often..
Eleanor#
Gloria Borgstahl wrote:
> Hello ccp4-ers,
>
> We are refining a 1.55 angstrom cryocooled structure with REFMAC.
> In analyzing the occupancy of ligands in the active site we noticed
> that all the residues there have bottomed out with an isotropic B value of
> 2.0.
> There is residual Fo-Fc density as the B values are not refined well.
> How can one fix this?
>
> Thanks, Gloria
>
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