(This question is repeatedly asked.)
Replace the "unit cell" parameters in the EM map file, like this:
mapman -b mapsize 8200000 << eof
read m1 bigmap.ccp4 ccp4
cell m1 749.8 749.8 749.8 90 90 90
write m1 smallmap.ccp4 ccp4
quit
eof
(for completeness, mapman originates in http://xray.bmc.uu.se/usf )
Happy Friday,
Dan
On Fri, 7 Aug 2009, Filip Van Petegem wrote:
> Hello all,
>
> While trying to perform some docking experiments with crystal structures
> into cryoEM maps, I found that some deposited EM maps are not at the right
> size relative to crystal structure coordinates (e.g. a ccp4 formatted
> deposited EM map looks smaller than a crystal structure that's supposed to
> represent less than 10% of the map). The problem is independent of the
> graphics program used (e.g. VMD, chimera, pymol all show the EM map as too
> small).
>
> Is there any straightforward way to get the map to the right scale, using
> information somehow hidden in the map header? Note that I don't know a
> priori by how much the EM map would have to be inflated. I'm using the
> SITUS package for docking - converting from ccp4 to situs, spider or any
> other common map format all deliver maps with the wrong size.
>
> Sincerely,
>
> Filip Van Petegem
>
>
>
>
>
--
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