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Hi, I have been recently trying to analysis multi-slice fMRI data using SPM96, and I was just wondering if my procedure has been correct. I have 5 adjacent slices which I first combine into volumes. Then, within SPM96, I realign and smooth the data but I don't normalise it (the slices I acquire are at an angle and so I cannot give an accurate origin for any normalisation). I model the data on a simple box-car function with no global normalisation. Is this sufficient analysis for such data?? Also, I have been attempting to overlay any activation I obtain on the subject's relevant anatomical slices (which have also been previously combined into a volume) using the 'slices' option. A problem which has arose for every dataset is that, when I do this, I only appear to have four anatomical slices regardless of whether there is activation or not. I'm not sure about the fifth slice and I was wondering if someone could explain why this has been happening. The 5 slices I acquire (both the anatomical and the functional) have a 0.5mm gap in-between. Is this the problem or is something inherent happening when I reslice the data to remove the final slice?? Thanks in advance, Carol. ---------------------- carol docherty [log in to unmask] %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%