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Hi, You should fix errors in the defoci first. Best regards, Takanori Nakane On 2019/03/26 22:03, Zhao, Haiyan wrote: > Thanks for your reply, I had another data set from same sample with 20 degree tilt, I got 4.4 A structure in relion, because preferred sample orientation, I need 40 degree tilt data to get better map. > Do you think that I put both data sets together and do bayesian polishing or I need do a couple of ctf refinements before bayesian polishing? > Best wishes, > > Haiyan Zha > Department of Biochemistry and Molecular Genetics > Voice: 858-869-3633 > > > > > > > > On 3/26/19, 3:34 PM, "Takanori Nakane" <[log in to unmask]> wrote: > >> Hi, >> >> At 14 A, there is no point running Polishing (yet) and >> you cannot reliably fit B factors. >> >> If you believe the resolution is limited by the defocus gradient, >> run several cycles of CtfRefine and Refine3D (see hemagglutinin example >> in our RELION3 paper) or use tilt-aware CTF estimation programs >> (e.g. Warp, CTFTILT, GoCTF). >> >> Best regards, >> >> Takanori Nakane >> >> On 2019/03/26 21:30, Zhao, Haiyan wrote: >>> Because it is a tilt 40 degree data set on K3 detector with 200KV Arctic, so far the resolution is 14A after 3D refinement >>> Resolution range is default, >>> Dose rate is 1.34 e-/A2, >>> >>> >>> Haiyan Zhao >>> Department of Biochemistry and Molecular Genetics >>> Voice: 858-869-3633 >>> >>> >>> >>> >>> >>> >>> >>> On 3/26/19, 3:17 PM, "Takanori Nakane" <[log in to unmask]> wrote: >>> >>>> Hi, >>>> >>>> What is the resolution of your structure? >>>> What is the resolution range you used to fit B factors? >>>> What is the dose rate? >>>> >>>> Best regards, >>>> >>>> Takanori Nakane >>>> >>>> On 2019/03/26 20:41, Zhao, Haiyan wrote: >>>>> I collected a tilt data set, when I run bayesian polishing for all >>>>> particles, I got the b factor like I attached the pdf file, I don’t know >>>>> what happened? Any suggestions. Thanks >>>>> >>>>> Haiyan Zhao >>>>> >>>>> Department of Biochemistry and Molecular Genetics >>>>> >>>>> Voice: 858-869-3633 >>>>> >>>>> >>>>> ------------------------------------------------------------------------ >>>>> >>>>> To unsubscribe from the CCPEM list, click the following link: >>>>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 >>>>> >>>> >> > > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1