Hi Amnah, This is an issue with oxford_asl when used with FSL6. In FSL6, FLIRT and APPLYWARP behave differently when the reference data is 4D - specifically they create an output data set with one volume for each reference volume. As a result, the corrected calibration data being fed into TOPUP does not have the expected dimensions. I've built an updated oxford_asl package (v3.9.22) which addresses this problem and runs correctly on the ASL primer data using TOPUP. You can get the update from the GitHub release page: https://github.com/ibme-qubic/oxford_asl/releases/tag/v3.9.22 There is also another issue you need to be aware of! In older versions of oxford_asl the --echospacing parameter behaved differently for fieldmap and TOPUP distortion correction. For fieldmap correction it was given as the true echo spacing (dwell time) whereas for TOPUP correction it had to be specified as the total readout time (echo spacing * number of slices in PE direction - 1). This has now been fixed so that the parameter means the same thing in both cases (the true echo spacing). In the ASL primer example that you're working from, the value has been given using the old convention and is therefore much too big! To correct it you need to divide it by 63 since the data size in the Y dimension is 64. This gives an echo spacing of approximately 0.00095s, so you should use --echospacing=0.00095. A line in the output should confirm how this value is being used, e.g. For TOPUP using total read-out time of (64 - 1) x 0.00095 s = .05985 s Hope this helps - please ask if you're unsure about the above, Martin ________________________________ From: FSL - FMRIB's Software Library <[log in to unmask]> on behalf of Amnah Mahroo <[log in to unmask]> Sent: 04 January 2019 10:06:12 To: [log in to unmask] Subject: [FSL] Topup Error while performing perfusion analysis using BASIL Hi FSL Experts, I have encountered Topup error report on ubuntu saying 'Sorry, the application applytop has stopped unexpectedly', while performing perfusion analysis using BASIL. The same message appears during both the command-line and the gui versions of BASIL. The analysis still goes on and produce the results as mentioned in the primer example box (http://www.neuroimagingprimers.org/examples/introduction-to-perfusion-quantification-using-asl/). Does this mean no distortion correction was applied during the analysis? Should I ignore this or fix this? The output from the run looks like this: /home/amnah/Downloads/oxford_asl/oxford_asl -i "/home/amnah/Desktop/ASL_Oxford_Primers_GUI/Example_2/singlePLDpcASL/asltc.nii.gz" --ibf=rpt --iaf=tc --tis 3.60 --bolus 1.80 --casl --slicedt 0.04520 -c "/home/amnah/Desktop/ASL_Oxford_Primers_GUI/Example_2/singlePLDpcASL/aslcalib.nii.gz" --tr 4.80 --cgain 1.00 --cmethod voxel --cblip="/home/amnah/Desktop/ASL_Oxford_Primers_GUI/Example_2/singlePLDpcASL/aslcalib_PA.nii.gz" --echospacing=0.06000 --pedir=y --wp --t1b 1.65 --alpha 0.85 --fixbolus --spatial --mc --artoff -o "oxasl" OXFORD_ASL - running Version: v3.9.21 Mon Nov 19 14:31:46 2018 Saving results in natve (ASL aquisition) space to oxasl/native_space Pre-processing Motion Correction Number of voxels is:98304 Number of repeats in data is:30 Outputting ASL data mean at each TI Done. Number of inversion times: 1 Number of timepoints in data: 30 Number of repeats in data: 30 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 3.60 Creating mask Distortion correction: running topup For TOPUP using total read-out time of ( 64 - 1) x 0.06000 s = 3.78000 s Part of FSL (ID: 6.0.0) topup Usage: topup --imain=<some 4D image> --datain=<text file> --config=<text file with parameters> --out=my_topup_results Compulsory arguments (You MUST set one or more of): --imain name of 4D file with images --datain name of text file with PE directions/times Optional arguments (You may optionally specify one or more of): --out base-name of output files (spline coefficients (Hz) and movement parameters) --fout name of image file with field (Hz) --iout name of 4D image file with unwarped images --logout Name of log-file --warpres (approximate) resolution (in mm) of warp basis for the different sub-sampling levels, default 10 --subsamp sub-sampling scheme, default 1 --fwhm FWHM (in mm) of gaussian smoothing kernel, default 8 --config Name of config file specifying command line arguments --miter Max # of non-linear iterations, default 5 --lambda Weight of regularisation, default depending on --ssqlambda and --regmod switches. See user documetation. --ssqlambda If set (=1), lambda is weighted by current ssq, default 1 --regmod Model for regularisation of warp-field [membrane_energy bending_energy], default bending_energy --estmov Estimate movements if set, default 1 (true) --minmet Minimisation method 0=Levenberg-Marquardt, 1=Scaled Conjugate Gradient, default 0 (LM) --splineorder Order of spline, 2->Qadratic spline, 3->Cubic spline. Default=3 --numprec Precision for representing Hessian, double or float. Default double --interp Image interpolation model, linear or spline. Default spline --scale If set (=1), the images are individually scaled to a common mean, default 0 (false) --regrid If set (=1), the calculations are done in a different grid, default 1 (true) -h,--help display help info -v,--verbose Print diagonostic information while running -h,--help display help info Topup: msg=topup_clp::topup_clp: Mismatch between /tmp/fsl_wW0Wyf_ox_asl/calib_blipped and /tmp/fsl_wW0Wyf_ox_asl/topup_params.txt Distortion Correction using TOPUP terminate called after throwing an instance of 'TOPUP::TopupFileIOException' what(): TopupFileIO:: msg=TopupFileReader::TopupFileReader: Unable to read file /tmp/fsl_wW0Wyf_ox_asl/topupresult /home/amnah/Downloads/oxford_asl/oxford_asl: line 1687: 2453 Aborted (core dumped) applytopup --imain=$tempdir/calib,$tempdir/cblip --inindex=1,2 --datain=$tempdir/topup_params.txt --topup=${topupresult} --out=$tempdir/calib --method=jac terminate called after throwing an instance of 'TOPUP::TopupFileIOException' what(): TopupFileIO:: msg=TopupFileReader::TopupFileReader: Unable to read file /tmp/fsl_wW0Wyf_ox_asl/topupresult /home/amnah/Downloads/oxford_asl/oxford_asl: line 1687: 2459 Aborted (core dumped) applytopup --imain=$tempdir/asldata --datain=$tempdir/topup_params.txt --inindex=1 --topup=${topupresult} --out=$tempdir/asldata --method=jac Number of voxels is:98304 Number of repeats in data is:30 Outputting ASL data mean at each TI Done. Setting up BASIL Instructing BASIL to use automated spatial smoothing Calling BASIL on data - conventional perusion image Creating output directory: /tmp/fsl_wW0Wyf_ox_asl/init/basil here STEP 1: VB - Tissue ---------------------- Welcome to FABBER v3.9.2-97-g3b6aec1 ---------------------- Last commit: Wed Sep 12 10:12:07 2018 Logfile started: /tmp/fsl_wW0Wyf_ox_asl/init/basil/step1/logfile 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%100% Final logfile: /tmp/fsl_wW0Wyf_ox_asl/init/basil/step1/logfile STEP 2: Spatial VB Tissue - init with STEP 1 ---------------------- Welcome to FABBER v3.9.2-97-g3b6aec1 ---------------------- Last commit: Wed Sep 12 10:12:07 2018 Logfile started: /tmp/fsl_wW0Wyf_ox_asl/init/basil/step2/logfile 0%5%10%15%20%25%30%35%40%45%50%55%60%65%70%75%80%85%90%95%100% Final logfile: /tmp/fsl_wW0Wyf_ox_asl/init/basil/step2/logfile End. Fabber variance calculator Working in /tmp/fsl_wW0Wyf_ox_asl/init/basil/step2 Calculating variance for: ftiss FABBER: MVNtool FabberRunDataNewimage::Loading mask data from '/tmp/fsl_wW0Wyf_ox_asl/mask' FabberRunDataNewimage::Dimensions: x=64, y=64, z=24, vols=1 FabberRunDataNewimage::Voxel size: x=3.4375mm, y=3.4375mm, z=4.95mm, TR=1 sec FabberRunDataNewimage::Intents: 0, 0, 0, 0 FabberRunDataNewimage::Setting coordinates from extent FabberRunDataNewimage::Loading data from '/tmp/fsl_wW0Wyf_ox_asl/init/basil/step2/finalMVN' FabberRunDataNewimage::Dimensions: x=64, y=64, z=24, vols=10 FabberRunDataNewimage::Voxel size: x=3.4375mm, y=3.4375mm, z=4.95mm, TR=1 sec FabberRunDataNewimage::Intents: 1005, 0, 0, 0 FabberRunDataNewimage::Applying mask to data... FabberRunData::GetVoxelData: input=/tmp/fsl_wW0Wyf_ox_asl/init/basil/step2/finalMVN mean value=1.13134e+06 FabberRunDataNewimage::Saving to nifti: /tmp/fsl_wW0Wyf_ox_asl/init/basil/step2/var_ftiss Calculating variance for: delttiss FABBER: MVNtool FabberRunDataNewimage::Loading mask data from '/tmp/fsl_wW0Wyf_ox_asl/mask' FabberRunDataNewimage::Dimensions: x=64, y=64, z=24, vols=1 FabberRunDataNewimage::Voxel size: x=3.4375mm, y=3.4375mm, z=4.95mm, TR=1 sec FabberRunDataNewimage::Intents: 0, 0, 0, 0 FabberRunDataNewimage::Setting coordinates from extent FabberRunDataNewimage::Loading data from '/tmp/fsl_wW0Wyf_ox_asl/init/basil/step2/finalMVN' FabberRunDataNewimage::Dimensions: x=64, y=64, z=24, vols=10 FabberRunDataNewimage::Voxel size: x=3.4375mm, y=3.4375mm, z=4.95mm, TR=1 sec FabberRunDataNewimage::Intents: 1005, 0, 0, 0 FabberRunDataNewimage::Applying mask to data... FabberRunData::GetVoxelData: input=/tmp/fsl_wW0Wyf_ox_asl/init/basil/step2/finalMVN mean value=1.13134e+06 FabberRunDataNewimage::Saving to nifti: /tmp/fsl_wW0Wyf_ox_asl/init/basil/step2/var_delttiss Done. Creating output directory: /tmp/fsl_wW0Wyf_ox_asl/basil here STEP 1: VB - Tissue - init with STEP 0 ---------------------- Welcome to FABBER v3.9.2-97-g3b6aec1 ---------------------- Last commit: Wed Sep 12 10:12:07 2018 Logfile started: /tmp/fsl_wW0Wyf_ox_asl/basil/step1/logfile 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%100% Final logfile: /tmp/fsl_wW0Wyf_ox_asl/basil/step1/logfile STEP 2: Spatial VB Tissue - init with STEP 1 ---------------------- Welcome to FABBER v3.9.2-97-g3b6aec1 ---------------------- Last commit: Wed Sep 12 10:12:07 2018 Logfile started: /tmp/fsl_wW0Wyf_ox_asl/basil/step2/logfile 0%5%10%15%20%25%30%35%40%45%50%55%60%65%70%75%80%85%90%95%100% Final logfile: /tmp/fsl_wW0Wyf_ox_asl/basil/step2/logfile End. Fabber variance calculator Working in /tmp/fsl_wW0Wyf_ox_asl/basil/step2 Calculating variance for: ftiss FABBER: MVNtool FabberRunDataNewimage::Loading mask data from '/tmp/fsl_wW0Wyf_ox_asl/mask' FabberRunDataNewimage::Dimensions: x=64, y=64, z=24, vols=1 FabberRunDataNewimage::Voxel size: x=3.4375mm, y=3.4375mm, z=4.95mm, TR=1 sec FabberRunDataNewimage::Intents: 0, 0, 0, 0 FabberRunDataNewimage::Setting coordinates from extent FabberRunDataNewimage::Loading data from '/tmp/fsl_wW0Wyf_ox_asl/basil/step2/finalMVN' FabberRunDataNewimage::Dimensions: x=64, y=64, z=24, vols=10 FabberRunDataNewimage::Voxel size: x=3.4375mm, y=3.4375mm, z=4.95mm, TR=1 sec FabberRunDataNewimage::Intents: 1005, 0, 0, 0 FabberRunDataNewimage::Applying mask to data... FabberRunData::GetVoxelData: input=/tmp/fsl_wW0Wyf_ox_asl/basil/step2/finalMVN mean value=0.606532 FabberRunDataNewimage::Saving to nifti: /tmp/fsl_wW0Wyf_ox_asl/basil/step2/var_ftiss Calculating variance for: delttiss FABBER: MVNtool FabberRunDataNewimage::Loading mask data from '/tmp/fsl_wW0Wyf_ox_asl/mask' FabberRunDataNewimage::Dimensions: x=64, y=64, z=24, vols=1 FabberRunDataNewimage::Voxel size: x=3.4375mm, y=3.4375mm, z=4.95mm, TR=1 sec FabberRunDataNewimage::Intents: 0, 0, 0, 0 FabberRunDataNewimage::Setting coordinates from extent FabberRunDataNewimage::Loading data from '/tmp/fsl_wW0Wyf_ox_asl/basil/step2/finalMVN' FabberRunDataNewimage::Dimensions: x=64, y=64, z=24, vols=10 FabberRunDataNewimage::Voxel size: x=3.4375mm, y=3.4375mm, z=4.95mm, TR=1 sec FabberRunDataNewimage::Intents: 1005, 0, 0, 0 FabberRunDataNewimage::Applying mask to data... FabberRunData::GetVoxelData: input=/tmp/fsl_wW0Wyf_ox_asl/basil/step2/finalMVN mean value=0.606532 FabberRunDataNewimage::Saving to nifti: /tmp/fsl_wW0Wyf_ox_asl/basil/step2/var_delttiss Done. Number of voxels is:35238 Number of repeats in data is:1 Start extrapolation! Done. Output is oxasl/ OXFORD_ASL - done. Return code: 0 I would really appreciate if someone can clarify this. Best Regards, Amnah ######################################################################## To unsubscribe from the FSL list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=FSL&A=1 ######################################################################## To unsubscribe from the FSL list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=FSL&A=1