Dear Experts, I'm trying to use MELODIC gui to run ICA on a series of activation likelihood estimation (ALE) maps. Hence, my input is a 4D nii where each volume is an unthresholded ALE map, and I'm treating it as a single subject, setting TR=1 (is this right?) Since data are not "real fmri" I'm not applying preprocessing. Related to this, which value should I set for high pass filter cut-off? I get the following error during Preprocessing:Stage 2 /usr/local/fsl/bin/fslstats prefiltered_func_data -p 2 -p 98 0.000000 0.004092 /usr/local/fsl/bin/fslmaths prefiltered_func_data -thr 0.0004092 -Tmin -bin mask -odt char /usr/local/fsl/bin/fslstats prefiltered_func_data -k mask -p 50 0.000000 /usr/local/fsl/bin/fslmaths mask -dilF mask /usr/local/fsl/bin/fslmaths prefiltered_func_data -mas mask prefiltered_func_data_thresh divide by zero while executing "if { [ catch { for { set argindex 1 } { $argindex < $argc } { incr argindex 1 } { switch -- [ lindex $argv $argindex ] { -I { incr arginde..." (file "/usr/local/fsl/bin/feat" line 312) Error encountered while running in main feat script, halting. divide by zero I checked the mask file and realized that is an all 0 file. So I manually performed this command /usr/local/fsl/bin/fslmaths prefiltered_func_data -thr 0.0004092 -Tmin -bin mask -odt char but removing the option -Tmin and the mask file now has values inside. However I'm not able to procede with the analyses. So my question is, if the error I get is really due to the -Tmin option, what could I do to solve it and run ICA without problems? Thank you for your help. Cheers, Jordi ######################################################################## To unsubscribe from the FSL list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=FSL&A=1