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Dear all, for anisotropic magnification you may also want to test Xmipp highres in Scipion. Kind regards, Carlos Oscar On 07/09/2018 17:32, Takanori Nakane wrote: > Hi, > > Magnification anisotropy correction and Ewald sphere correction are > different concepts. > > Unfortunately RELION 3.0 does not support magnification anisotropy > estimation and correction. > We are working for it but it will not be included in version 3.0. > > For Ewald sphere correction, please use relion_reconstruct: > > relion_reconstruct --i run_data.star --o half1.mrc --subset 1 --angpix 1.23 > --ctf --ewald --mask_diameter 560 --sectors 2 --width_mask_edge 5 --sym I1 > > Then repeat this for the other half (--subset 2) as well. > > See the paper (https://doi.org/10.1016/j.ultramic.2017.11.001) > for the meanings of mask_diameter, sectors and width_mask_edge. > > Best regards, > > Takanori Nakane > >> Dear Community, >> >> Has anyone had any experience with applying ewald sphere correction in >> relion 3.0? >> I understand that this feature is still in beta but I would like to give >> it >> a go. Would be helpful to have some guidance on its usage. >> Specifically, does the program automatically calculate the magnification >> anisotropy *a la* jspr or does it need to be determined at the scope and >> then the correct values applied during the reconstruction? >> >> Cheers, >> pranav >> >> -- >> Pranav Shah >> Postdoctoral Research Fellow. >> >> Hogle Lab >> Harvard Medical School >> 240 Longwood Avenue >> Boston, MA 02115 >> (617) 432-4360 (fax) >> (617) 432-3839 (lab) >> >> ######################################################################## >> >> To unsubscribe from the CCPEM list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 >> > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > -- ------------------------------------------------------------------------ Carlos Oscar Sánchez Sorzano e-mail: [log in to unmask] Biocomputing unit http://i2pc.es/coss National Center of Biotechnology (CSIC) c/Darwin, 3 Campus Universidad Autónoma (Cantoblanco) Tlf: 34-91-585 4510 28049 MADRID (SPAIN) Fax: 34-91-585 4506 ------------------------------------------------------------------------ ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1