Unfortunately,

The problem here lies primarily in the answer given,  not so much in the question asked by a newcomer:

"1) In cryo-EM maps are not electron density maps but surfaces representing electric potential. "

The answer appears to reflect the widespread misunderstanding that EM images (and hence cryo-EM maps)  only show the surfaces not the internal density of the complexes we study.
In my Imperial College/Leiden University  lecture notes, I have always used the below slide to illustrate this point.

Cheers,

Marin

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On 10/09/2018 01:38, Pavel Afonine wrote:

Hi,

Is there any sever available to create electron density maps for cryo-em structures?

The questions are nonsensical. Here is why:

1) In cryo-EM maps are not electron density maps but surfaces representing electric potential.

2) Creating such a map is essentially carrying on from cryo-EM experiment and obtaining the 3D reconstruction.

Are you really sure about what you are asking for?

 

Or, we should create the maps from mmCIF.

mmCIF is a file format. It may contain representations of rabbits, boysenberries or some diffraction data. So.. how you think it may be related to cryo-EM, in your particular case?

 

I am particularly interested in those cryo-em structures with high resolution, like 2.6~2.8A. 

Sure, all are excited about high-res cryo-EM!!! 

 

Please give me an education.

Sure. One of available universities can do this.

Cheers,

Pavel

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    Prof Dr Ir Marin van Heel

    Laboratório Nacional de Nanotecnologia - LNNano
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    Leiden University
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    Imperial College London
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