 |
 |
Hi, RELION-3's re-implementation of the MotionCor2 algorithm masks hot pixels but ignores the defect file. Indeed this might bias the trajectory to the origin, but I would not worry as long as you run Bayesian Polishing afterwards; the convergence radius of Bayeisan Polishing is large. Bayesian Polishing compares particles with reference projections. Thus, the effect of fixed pattern noise is smaller than in reference-free methods. Best regards, Takanori Nakane > Hi, > > Can RELION-3 deal with outlier pixels (bright pixels) that may affect > drift correction in the early parts of data processing? I know this can be > done in cisTEM. > > Best wishes, > > Noor > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1