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Hi all, We have been using UltrAufoil for a few weeks now with great success in terms of stability and reproducibility. However also reproducible are gold particles in the holes (see picture of an extreme case). These are certainly great for tomography not so much for SPA. Anyway is there a specific way to clean these grids in order to get rid of the unwanted little buggers? We usually glow discharge our grids with the Pelco easyglow system and its default settings in air, one side only. Regards Eric ----------------- Assoc. Prof Eric Hanssen Head - Advanced Microscopy Facility Honorary Principal Fellow - Department of Biochemistry and Molecular Biology Bio21 Molecular Science and Biotechnology Institute 30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia email: [log in to unmask]<mailto:[log in to unmask]> | Office: +61 3 83442449 | Microscope: +61 3 83442509 Web: www.microscopy.unimelb.edu.au<http://www.microscopy.unimelb.edu.au/> [qqwd] ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1