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Hi, Robb McLeod studies this problem in detail, and came up with the MRCZ standard, as described at https://doi.org/10.1016/j.jsb.2017.11.012 Or https://www.biorxiv.org/content/early/2017/03/13/116533 The programs are available at https://github.com/em-MRCZ Henning. Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62 On Jun 3, 2018, at 3:15 PM, Oliver Clarke <[log in to unmask]<mailto:[log in to unmask]>> wrote: I would vote for bzip2 over compressed tiff. For a test 50 frame, 1.4G stack, pbzip2 gives a 214M archive, while mrc2tif gives a 264M tiff - and pbzip2 is much faster to compress/decompress at least on my system (GPU workstation with 24 cores/48 threads) Cheers Oli On Jun 3, 2018, at 9:00 AM, Dimitry Tegunov <[log in to unmask]<mailto:[log in to unmask]>> wrote: Hey! In addition to what Takanori wrote: If you're stuck with EPU for data collection, this tool can run on the K2 computer to convert gain-uncorrected MRCs to compressed TIFFs on-the-fly: https://github.com/dtegunov/stacker2. It will also happily compress your old files, assuming they contain only integers. I very much hope that one day we will get an EPU version with TIFF support for K2/3 data. And with most of SerialEM's functionality. And a pony. In this exact order, please ;-) Cheers, Dimitry ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1